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РЖ ВИНИТИ 76 (BI29) 96.06-04Н1.84

Feng, Z. -M.
Negative control of the rat inhibin 'альфа' subunit promoter in MA-10 Leydig tumour cells [Text] / Z. -M. Feng, C. -L.C. Chen // J. Mol. Endocrinol. - 1994. - Vol. 13, N 1. - P39-47 . - ISSN 0952-5041
Перевод заглавия: Негативный контроль промотора 'альфа'-субъединицы[гена] ингибина крысы в опухоли из клеток Лейдига линии MA-10
Аннотация: The promoter/regulatory sequences responsible for the transcription of the rat inhibin 'альфа' subunit gene in the testis were identified by the transient expression in an MA-10 Leydig tumour cell line of a bacterial reporter gene, chloramphenic0l acetyltransferase (CAT), which was driven by different regions of the 5' flanking sequence of the inhibin 'альфа' subunit gene. The CAT activity was elevated when the 2*0 kb 5' flanking 'альфа' subunit gene fragment was progressively shortened from its 5' end, and a maximal increase was reached when the CAT gene was driven by an 'альфа' subunit gene promoter extending to - 163 bp. This construct was termed A'альфа'BstCAT. Furthermore, when either the - 2* 0 to - 1* 6 kb or the - 2*0 to - 1* 0 kb 'альфа' subunit DNA fragment was fused to A'альфа'BstCAT, the CAT activity was markedly suppressed, indicating the presence of negative regulatory DNA elements (NRE[s]) in the upstream region of the gene. The cyclic AMP (cAMP) responsiveness of the 'альфа' subunit gene, which was dependent upon the putative cAMP response element within the 67 bp 'альфа' subunit promoter, was not effected by the upstream NREs. The inhibitory effect was also demonstrated when the - 2*0 to - 1* 0 kb fragment was placed in either orientation with, respect to the 'альфа' subunit promoter or to a thymidine kinase promoter, suggesting that the NRE(s) can act as a silencer. We conclude that the basal expression of the rat inhibin 'альфа' subunit gene in testicular MA-10 cells may, at least in part, be controlled by the upstream silencer(s) and NRE(s). США, Population Council, 1230 York Avenue, New York, New York 10021, USA. Библ. 37

ГРНТИ	76.29.49

ВИНИТИ 761.29.49.15.19
Рубрики: ИНГИБИН
СУБЪЕДИНИЦА АЛЬФА
ПРОМОТОР РЕКОМБИНАНТНЫЙ
ЭКСПРЕССИЯ ВРЕМЕННАЯ
НЕГАТИВНЫЙ РЕГУЛЯТОРНЫЙ ЭЛЕМЕНТ NRE
ВЫЯВЛЕНИЕ
ЛОКАЛИЗАЦИЯ
ТРАНСКРИПЦИЯ
РЕГУЛЯЦИЯ
КРЫСЫ
ОПУХОЛЬ ЛЕЙДИГА
КЛЕТКИ ЛИНИИ MA-10

Доп.точки доступа:
Chen, C.-L.C.

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