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РЖ ВИНИТИ 34 (BI39) 95.11-04К1.392

Mertsching, Elisabeth.
IL-7 transgenic mice: Analysis of the role of IL-7 in the differentiation of thymocytes in vivo and in vitro [Text] / Elisabeth Mertsching, Corinne Burdet, Rhodri Ceredig // Int. Immunol. - 1995. - Vol. 7, N 3. - P401-414 . - ISSN 0953-8178
Перевод заглавия: Трансгенные по интерлейкину (ИЛ) 7 мыши. Анализ роли ИЛ-7 в дифференцировке тимоцитов in vivo и in vitro
Аннотация: We have generated a high copy number transgenic mouse line in which expression of mouse IL-7 cDNA is under the control of the mouse MHC class II E'альфа' promoter. These mice were generated in order to see if IL-7 over-production in the thymus altered either thymocyte differentiation or the process of negative selection. Using in situ hybridization, IL-7 transcripts could be detected in the thymic cortex and medulla as well as the spleen and lymph nodes of transgenic mice but was undetectable in normal controls. Phenotypic and molecular analysis of thymocytes from embryonic and adult transgenic mice failed to reveal a dramatic effect of IL-7 on thymocyte differentiation and negative selection of the TCR V['бета'] repertoire appeared to be intact. In peripheral lymph nodes, there was a massive (30-fold) increase in the number of T cells (CD8{+} CD4{+}) and simultaneous presence of immature (B220{+}, Ig{-}) B cells. TCR repertoire analysis showed that the expansion of peripheral T cells was polyclonal. Using the polymerase chain reaction (PCR), transgene-specific IL-7 transcripts could be detected in the thymus from day 14 of fetal development. However, using semi-quantitative PCR, there was no dramatic increase in the degree of TCR'бета' or TCR'альфа' gene rearrangements during thymocyte ontogeny in vivo. Similarly, when fetal mouse thymus lobes were cultured with IL-7 in vitro, there was no dramatic increase in the degree of TCR'бета' or TCR'альфа' gene rearrangements. We conclude that IL-7 is probably not an important differentiation factor for immature mouse thymocytes. Франция, Inst. Chemie Biol., Med. Fac., Strasbourg. Библ. 41

ГРНТИ	34.43.35

ВИНИТИ 341.43.35.15.05
Рубрики: ИНТЕРЛЕЙКИН 7
ТИМОЦИТЫ
ДИФФЕРЕНЦИРОВКА
МЕХАНИЗМЫ
ТРАНСГЕННЫЕ МЫШИ

Доп.точки доступа:
Burdet, Corinne; Ceredig, Rhodri

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