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РЖ ВИНИТИ 34 (BI39) 96.04-04К1.203

Human HLA-A0201-restricted cytotoxic T lymphocyte recognition of influenza A is dominated by T cells bearing the V'бета'17 gene segment [Text] / Paul J. Lehner [et al.] // J. Exp. Med. - 1995. - Vol. 181, N 1. - P79-91 . - ISSN 0022-1007
Перевод заглавия: Распознавание вируса гриппа A ограниченными по HLA-A0201 цитотоксическими T-лимфоцитами человека происходит преимущественно T-клетками, имеющими сегмент гена V'бета' 17
Аннотация: The major histocompatibility complex class I-restricted cytotoxic T lymphocyte (CTL) response is important in the clearance of viral infections in humans. After influenza A infection, a peptide from the matrix protein, M58-66, is presented in the context of the MHC allele HLA-A0201 and the resulting CTL response is detectable in most HLA-A0201 subjects. An initial study suggested that M58-66-specific CTL clones show conserved T cell receptor (TCR) 'альфа' and 'бета' gene segments. Were addressed the significance of this observation by determining the expression of V'бета'17 during the development of M58-66-specific CTL lines in 21 unrelated HLA-A0201 subjects, and analyzing TCR usage by M58-66-specific CTL clones. TCR V'бета'17 was the dominant V'бета' segment used and CD8 V'бета'17 expansion correlated with M58-66-specific lysis. Limiting dilution analysis from five subjects showed the M58-66 CTL precursor frequency to vary between 1/54,000 and less than 1/250,000, and that up to 85% of the matrix peptide (M58-66)-specific CTL used the V'бета'17 gene segment. The M58-66 specific CTL response was dependent on previous viral exposure and specific V'бета'17 expansion, as it was not found in cord blood, despite a readily expandable V'бета'17{+} CD8{+} T cell subpopulation. Sequence analysis of 38 M58-66-specific V'бета'17 transcripts from 13 subjects revealed extensive conservation in the CDR3 region including conservation of an arginine-serine motif. To test the dependence of this CTL response on the V'бета'17 gene segment, peripheral blood lymphocytes were depleted of CD8{+} TCR V'бета'17{+} cells, before the generation of M58-66-specific CTL. Such depletion blocked or severely reduced the generation of the M58-66-specific response, and under limiting dilution conditions could abolish M58-66-specific CTL precursors. Великобритания, Dep. Med., Univ. Wales Med. College, Cardiff. Библ. 52

ГРНТИ	34.43.29

ВИНИТИ 341.43.29.11
Рубрики: ЦИТОТОКСИЧЕСКИЕ ЛИМФОЦИТЫ Т
РАСПОЗНАВАНИЕ ВИРУСНОГО АНТИГЕНА
МОЛЕКУЛА HLA-A 0201 ГКГС
ЧЕЛОВЕК
ВИРУС ГРИППА

Доп.точки доступа:
Lehner, Paul J.; Wang, Eddie C.Y.; Moss, Paul A.H.; Williams, Sheila; Platt, Kaye; Friedman, Steven M.; Bell, John I.; Borysiewicz, Leszek K.

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