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РЖ ВИНИТИ 34 (BI38) 96.07-04А3.310

Mehta, Shreefal.
Effects of high levels of fluoride on bone formation: An in vitro model system [Text] / Shreefal Mehta, Berenice Reed, Peter Antich // Biomaterials. - 1995. - Vol. 16, N 2. - P97-102 . - ISSN 0142-9612
Перевод заглавия: Влияние высокого содержания фторидов на образование кости. Модельная система in vitro
Аннотация: In order to develop an in vitro model for the study of the effects of different agents on biomineralization, a three-dimensional cell culture system was investigated at different levels of fluoride. Rat fetal osteoblasts were seeded onto collagen discs and maintained in a culture medium for 40 days. Results showed that, at 40 days, the cultured matrices had a Ca:P ratio, mineral content and Fourier transform infrared (FTIR) spectrum that were close to those seen for normal rat bone. Viable cells, observed by light microscopy, were present in the matrix at 40 days. The formation of a mineralized matrix in this experimental set-up provided a model for exploring in vitro the effects of high levels of fluoride on bone. The fluoride content of the mineral formed in the cultures showed a dose-dependent increase in fluoride content with time. Also, an increase in the crystallinity of the apatite in the presence of fluoride, was observed by FTIR. The Ca:P ratio and percentage mineral by weight showed no apparent differences among the groups. The three-dimensional model used for this study has the potential to be a powerful tool in the study of time-dependent effects of drugs and other factors on osteoblast cell functions and subsequently on matrix mineralization. США, Advanced Radiological Sciences. Dep. of Radiology, Univ. of Texas Southwestern Med. Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75235-9058. Ил. 5. Библ. 36

ГРНТИ	34.57.21

ВИНИТИ 341.57.21
Рубрики: БИОМАТЕРИАЛЫ
КОСТИ
ОБРАЗОВАНИЕ
МАТРИЦА
ОСТЕОБЛАСТЫ
КОЛЛАГЕН
ФТОРИДЫ
ТОКСИЧНОСТЬ
ТРЕХМЕРНАЯ СИСТЕМА КУЛЬТИВИРОВАНИЯ
ФУРЬЕ ПРЕОБРАЗОВАНИЕ
ИК-СПЕКТРОСКОПИЯ

Доп.точки доступа:
Reed, Berenice; Antich, Peter

РЖ ВИНИТИ 34 (BI26) 96.08-04М4.993

Mehta, Shreefal.
Effects of high levels of fluoride on bone formation: An in vitro model system [Text] / Shreefal Mehta, Berenice Reed, Peter Antich // Biomaterials. - 1995. - Vol. 16, N 2. - P97-102 . - ISSN 0142-9612
Перевод заглавия: Влияние высокого содержания фторидов на образование кости. Модельная система in vitro
Аннотация: In order to develop an in vitro model for the study of the effects of different agents on biomineralization, a three-dimensional cell culture system was investigated at different levels of fluoride. Rat fetal osteoblasts were seeded onto collagen discs and maintained in a culture medium for 40 days. Results showed that, at 40 days, the cultured matrices had a Ca:P ratio, mineral content and Fourier transform infrared (FTIR) spectrum that were close to those seen for normal rat bone. Viable cells, observed by light microscopy, were present in the matrix at 40 days. The formation of a mineralized matrix in this experimental set-up provided a model for exploring in vitro the effects of high levels of fluoride on bone. The fluoride content of the mineral formed in the cultures showed a dose-dependent increase in fluoride content with time. Also, an increase in the crystallinity of the apatite in the presence of fluoride, was observed by FTIR. The Ca:P ratio and percentage mineral by weight showed no apparent differences among the groups. The three-dimensional model used for this study has the potential to be a powerful tool in the study of time-dependent effects of drugs and other factors on osteoblast cell functions and subsequently on matrix mineralization. США, Advanced Radiological Sciences. Dep. of Radiology, Univ. of Texas Southwestern Med. Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75235-9058. Ил. 5. Библ. 36

ГРНТИ	34.39.47

ВИНИТИ 341.39.47.09
Рубрики: БИОМАТЕРИАЛЫ
КОСТИ
ОБРАЗОВАНИЕ
МАТРИЦА
ОСТЕОБЛАСТЫ
КОЛЛАГЕН
ФТОРИДЫ
ТОКСИЧНОСТЬ
ТРЕХМЕРНАЯ СИСТЕМА КУЛЬТИВИРОВАНИЯ
ФУРЬЕ ПРЕОБРАЗОВАНИЕ
ИК-СПЕКТРОСКОПИЯ

Доп.точки доступа:
Reed, Berenice; Antich, Peter

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