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РЖ ВИНИТИ 34 (BI39) 95.09-04К1.248

Kuruvilla, Abraham.
Platelet-activating factor induces the tyrosine phosphorylation and activation of phospholipase C-'гамма'1, Fyn and Lyn kinases, and phosphotidylinositol 3-kinase in a human B cell line [Text] / Abraham Kuruvilla, Christian Pielop, William T. Shearer // J. Immunol. - 1994. - Vol. 153, N 12. - P5433-5442 . - ISSN 0022-1767
Перевод заглавия: Фактор, ассоциированный с тромбоцитами, вызывает фосфорилирование тирозина и активацию фосфолипазы C-'гамма'1, Fyn и Lyn киназ и фосфатидилинозитол 3-киназы в В-клетках человека
Аннотация: Platelet-activating factor (PAF) is a versatile lipid mediator of inflammation in a vfriety of biologic systems. We have previously reported that one of the earliest events in the signal transductions pathway of PAF in a human B lymphoblastoid cell line was the induction of tyrosine kinase activity concomitant with the activation of phospholipase C (PLC). We now demonstrate the occurrence of multiple tyrosine phosphorylation-dependent events which follow the interaction of PAF with its receptor of B cells. Anti-phosphotyrosine immunoprecipitates from lysates of PAF-stimulated cells, when fractionated by SDS-PAGE and analyzed by Western blotting with anti-PLC-'гамма'1, showed that maximal tyrosine phosphorylation of this enzyme occurred within 2 min of stimulation. This phenomenon was verified by immunoprecipitating with anti-PLC-'гамма'1 and subsequently probing with anti-phosphotyrosine. Immunoprecipitation of the tyrosine kinases, Fyn and Lyn, from PAF-stimulated cells, and use of these immunoprecipitates in kinase assays established that the activation of both kinases also occurred within the first 2 min of stimulation with phosphorylation occurring on their tyrosine residues. Additionally, we wlso provide evidence for the tyrosine phosphorylation of the p85 subunit of phosphatidylinositol 3-kinase (Ptdlns 3-kinase) and activation of this kinase by PAF in a dose-dependent manner, maximal activation occurring within 10 min poststimulation. We have thus demonstrated that the activation of tyrosine kinases is an important proximate step in PAF-midiated signal transduction in B cells, leading to tyrosine phosphorylation and activation of PLC-'гамма'1, Fyn and Lyn kinases, and Ptdlns 3-kinase. США, Dep. Pediatrics, Baylor College Med., Houston, TX 77030. Библ. 62

ГРНТИ	34.43.35

ВИНИТИ 341.43.35.15.11
Рубрики: ЛИМФОЦИТЫ B
КУЛЬТИВИРУЕМЫЕ ЛИНИИ
ФОСФОРИЛИРОВАНИЕ ТИРОЗИНА
ФАКТОР АКТИВАЦИИ ТРОМБОЦИТОВ
ФОСФОЛИПАЗЫ
ФОСФАТИДИЛИНОЗИТОЛКИНАЗА 3

Доп.точки доступа:
Pielop, Christian; Shearer, William T.

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