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РЖ ВИНИТИ 34 (BI39) 96.03-04К1.88

Polymenis, Michael.
Domain interactions and antigen binding of recombinant anti-Z-DNA antibody variable domains: The role of heavy and light chains measured by surface plasmon resonanse [Text] / Michael Polymenis, B.David Stollar // J. Immunol. - 1995. - Vol. 154, N 5. - P2198-2208 . - ISSN 0022-1767
Перевод заглавия: Междоменное взаимодействие и связывание антигенов рекомбинантными вариабельными доменами антител к Z-ДНК. Роль тяжелых и легких цепей, определяемая поверхностным плазменным резонансом
Аннотация: The heavy (H) and light (L) chain V domains of anti-Z-DNA mouse mAb Z22 were expressed separately in bacteria. When mixed in vitro, the V domains associated stoichiometrically to reconstitute the Ag binding site of Z22, as judged by specific reactivity with Z-DNA and anti-Z22 Id Abs. The apparent K[d] of the Z22 V[H]-V[L] association was 5.47*10{-8} M, measured by surface plasmon resonance. A replacement at V[L] position 96, which reduced Ag binding affinity of a single chain Fv (clone LZ1-2) by two orders of magnitude, did not reduce the affinity of interaction between the V[H] and V[L] domains (apparent K[d] = 1.93*10{-8} M for V[H] association with LZ1-2). Fab prepared from native Z22 bound specifically to a 30-bp Z-DNA oligonucleotide with an apparent K[d] = 1.56*10{-8} M. The V[H] domain alone bound Z-DNA specifically with an affinity similar to that of the Fab or Fv's of Z22 (K[d] = 1.68*10{-8}M), whereas Z22 V[L] domain alone did not interact with nucleic acids. Z22 V[H] binding to Ag was inhibited by association with the mutant LZ1-2 V[L]. These results indicate that the Z22 H chain makes important contributions to specific binding of Z-DNA. Although the L chain does not add greatly to the binding energy, an appropriate L chain is required to permit Ag binding in the Fv domain. These in vitro results resemble the in vivo modulation of H chain autoreactivity that occurs with L chain substitution in receptor editing. США, Dep. Biochem., Tufts Univ. Sch. Med., 02111 Boston. Библ. 55

ГРНТИ	34.43.33

ВИНИТИ 341.43.33.05
Рубрики: АНТИТЕЛА
К Z-ДНК
РЕКОМБИНАНТНЫЕ
ВАРИАБЕЛЬНЫЕ ДОМЕНЫ
СВЯЗЫВАНИЕ АНТИГЕНА

Доп.точки доступа:
Stollar, B.David

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