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РЖ ВИНИТИ 76 (BI29) 95.12-04Н1.272

Smart, Darren.
'мю'-Opioids activate phospholipase C in SH-SY5Y human neuroblastoma cells via calcium-channel opening [Text] / Darren Smart, Graham Smith, David G. Lambert // Biochem. J. - 1995. - Vol. 305, N 2. - P577-582 . - ISSN 0264-6021
Перевод заглавия: 'мю'-Опиоид активирует фосфолипазу С в SH-SY5Y клетках нейробластомы через открытие Ca каналов
Аннотация: We have recently reported that, in SH-SY5Y cells, 'мю'-opioid receptor occupancy activates phospholipase C via a pertussis toxin-sensitive G-protein. In the present study we have further characterized the mechanisms involved in this process. Fentanyl (0.1 'мю'M) caused a monophasic increase in inositol 1,4,5-trisphosphate mass formation, with a peak (20.5'+-'3.6 pmol/mg of protein) at 15 s. Incubation in Ca{2+}-free buffer abolished this response, while Ca{2+} replacement 1 min later restored the stimulation of inositol 1,4,5-trisphosphate formation (20.1'+-'0.6 pmol/mg of protein). In addition, nifedipine (1 nM-0.1 mM), an L-type Ca{2+}-channel antagonist, caused a dose dependent inhibition of inositol 1,4,5-trisphosphate formation, with an IC[50] of 60.3'+-'1.1 nM. Elevation of endogenous 'бета'/'гамма' subunits by selective activation of 'дельта'-opioid and 'альфа'[2] adrenoceptors failed to stimulate phospholipase C. Fentanyl also caused a dose-dependent (EC[50] of 16.2'+-'1.0 nM), additive enhancement of carbachol-induced inositol 1,4,5-trisphosphate formation. In summary, we have demonstrated that in SH-SY5Y cells activation of the 'мю'-opioid receptor allows Ca{2+} influx to activate phospholipase C. However, the possible role of this mechanism in the process of analgesia remains to be elucidated. Великобритания, Univ. Dep. Anaesthesia, Leicester Royal Infirmary, Leicester LE1 5WW. Библ. 45

ГРНТИ	76.29.49

ВИНИТИ 761.29.49.19.11.21
Рубрики: КАЛЬЦИЕВЫЕ КАНАЛЫ
ФОСФОЛИПАЗА С
АКТИВАЦИЯ 'МЮ'-ОПИОИДОМ
КЛЕТКИ SH-SY5Y
НЕЙРОБЛАСТОМА

Доп.точки доступа:
Smith, Graham; Lambert, David G.

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