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РЖ ВИНИТИ 34 (BI16) 96.01-04В3.152

    Becker, Roswitha.

    Subcellular localization and characterization of excessive iron in the nicotianamine-less mutant chloronerva [Text] / Roswitha Becker, Eberhard Fritz, Renate Manteuffel // Plant Physiol. - 1995. - Vol. 108, N 1. - P269-275 . - ISSN 0032-0889
Перевод заглавия: Субклеточная локализация и [метаболизм] избытка железа в мутантных растениях Lycopersicon esculentum chloronerva с низким содержанием никотинамина
Аннотация: To understand the function of the Fe{2+}-complexing compound nicotianamine (NA) in the iron metabolism of plants we have localized iron and other elements in the NA-containing tomato wild type (Lycopersicon esculentum) and its NA-free munant chloronerva by quantitative x-ray microanalysis. Comparison of element composition of the rhizodermal cell walls indicated that the wild type accumulated considerable amounts of iron and phosphorus in the cell wall, whereas in the munant irom and phosphorus were detected in the cytoplasm and vacuoles of the rhizodermis. In mutant leaves containing high iron concentrations in the symplast, electron-dense inclusions were detected in chloroplasts and phloem. Such particles, consisting mainly of iron and phosphorus, were never found in the wild type and were very rarely detected in young chlorotic munant leaves or after treatment of the mutant with NA. For further characterization the electron-dense inclusions in munant leaves were isolated and compared by sodium dodecyl sulfate-gel electrophoresis and immunoblotting to ferritin from iron-loaded Phaseolus vulgaris leaves. Antibodies raised against purified Phaseolus leaf ferritin were used. Neither in munant nor in wild type (iron loaded and control) was ferritin protein detected. These results suggest that the electron-dense inclusions in munant leaves are not identical with ferritin. It is concluded thah NA is necessary to complex ferrous iron in a soluble and available form within the cells. In the absence of NA the precipitation of excessive iron in the form of insoluble ferric phosphate compounds could protect the cells from iron overload. Библ. 47
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ВИНИТИ 341.31.21.15.19
Рубрики: ЖЕЛЕЗО
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Доп.точки доступа:
Fritz, Eberhard; Manteuffel, Renate

 




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