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РЖ ВИНИТИ 34 (BI13) 96.05-04Б3.85

Kim, In Seop.
Kinetic study on the production and degradation of leupeptiin in Streptomyces exfoliatus SMF13 [Text] / In Seop Kim, Kye Joon Lee // J. Biotechnol. - 1995. - Vol. 42, N 1. - P35-44 . - ISSN 0168-1656
Перевод заглавия: Изучение кинетики продукции и деградации леупептина в Streptomyces exfoliatus SMF13
Аннотация: Medium composition and cultivation conditions were constructed for the optimum production of leupeptin by Streptomyces exfoliatus SMF13. The production of leupeptin was related to mycelial growth, being optimum in the cultivation with glucose-excess, phosphate-limited, and casamino acids medium. However, leupeptin-inactivating enzyme (LIE) was produced in the cultivation with glucose-limited, phosphate-excess, and Na-caseinate medium where mycelium degradation was accompanied. Optimum production process of leupeptin was determined with phosphate-limited continuous cultivation, which did not permit LIE production. The maximum productivity (0.24 g 1{-1} h{-1}) and production yield (1.64 g leupeptin per g glucose) of phosphate-limited chemostat cultivation were 2.4- and 4-times larger than those of batch cultivation, respectively. This is the first cultivation kinetic analysis for leupeptin production and its inactivation by LIE in relation to mycelium differentiation. Южная Корея, Dept Microbiol., Coll. Nat. Sci., Res. Cent. Mol. Microbiol., Seoul Nat. Univ., Seoul 151-742. Библ. 23

ГРНТИ	34.27.39

ВИНИТИ 341.27.39.09.25
Рубрики: ИНГИБИТОРЫ
ИНГИБИТОРЫ ПРОТЕАЗ
ЛЕУПЕПТИН
ПОЛУЧЕНИЕ
ДЕГРАДАЦИЯ
КИНЕТИКА ПРОЦЕССОВ
STREPTOMYCES EXFOLIATUS (BACT.)
ШТАММ SMF 13

Доп.точки доступа:
Lee, Kye Joon

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