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РЖ ВИНИТИ 34 (BI23) 96.10-04М2.228

Expression of the subtype 1A dopamine receptor in the rate heart [Text] : [Pap.] 49th Annu. Fall Conf. and Sci. Sess. Counc. High Blood Pressure Res., New Orleans, La, Sept. 16-22, 1995 / Ryoji Ozono [et al.] // Hypertension. - 1996. - Vol. 27, N 3. - P693-703 . - ISSN 0194-911X
Перевод заглавия: Экспрессия субтипа 1А дофаминового рецептора в сердце крыс
Аннотация: In the present study using light microscopic immunohistochemistry, electron microscopic immunocytochemistry, and in situ amplification of mRNA, we demonstrate the D[1A] receptor in Sprague-Dawley and Wistar-Kyoto rat hearts. Selectivity was validated by recognition of the D[1A] receptor expressed in stably transfected LTK{-} cells. D[1A] receptor mRNA was detected with a novel transcription-based isothermal in situ amplification system as well as with reverse transcription-polymerase chain reaction. D[1A] receptor protein was distributed throughout theatrium and ventricular myocardium. Preimmune and preabsorption controls were negative. Electron microscopic immunocytochemistry using the protein A gold method demonstrated the D[1A] receptor along the cellular membranes of coronary smooth muscle cells and ventricular myocytes and in the myosin thick filaments and M-lines. D[1A] receptor mRNA was present in coronary vessels and myocardium in amplified but not in unamplified sections. Western blot analysis showed specific D[1A] bands in transfected LTK{-} cells and the atrium but not in nontransfected LTK{-} cells and the ventricle. The selective L1-like receptor agonist SKF38393 stimulated adenylyl cyclase in ventricular myocardial plasma membranes in a dose-related fashion, and the response was abolished by the selective D1-like receptor antagonist SCH23390. These results demonstrate that the D[1A] receptor gene and protein are expressed in normal rat heart. The physiological and pathophysiological roles and predominant cell signaling mechanism or mechanisms of this receptor remain to be determined

ГРНТИ	34.39.29

ВИНИТИ 341.39.29.11.02
Рубрики: СЕРДЦЕ
ДОФАМИНОВЫЕ РЕЦЕПТОРЫ
СУБТИП 1А
ЭКСПРЕССИЯ
КРЫСЫ

Доп.точки доступа:
Ozono, Ryoji; O'Connell, Damian P.; Vaughan, Carl; Botkin, Suzanne J.; Walk, Scott F.; Felder, Robin A.; Carey, Robert M.

РЖ ВИНИТИ 34 (BI49) 96.12-04М1.400

Expression of the subtype 1A dopamine receptor in the rate heart [Text] : [Pap.] 49th Annu. Fall Conf. and Sci. Sess. Counc. High Blood Pressure Res., New Orleans, La, Sept. 16-22, 1995 / Ryoji Ozono [et al.] // Hypertension. - 1996. - Vol. 27, N 3. - P693-703 . - ISSN 0194-911X
Перевод заглавия: Экспрессия субтипа 1А дофаминового рецептора в сердце крыс
Аннотация: In the present study using light microscopic immunohistochemistry, electron microscopic immunocytochemistry, and in situ amplification of mRNA, we demonstrate the D[1A] receptor in Sprague-Dawley and Wistar-Kyoto rat hearts. Selectivity was validated by recognition of the D[1A] receptor expressed in stably transfected LTK{-} cells. D[1A] receptor mRNA was detected with a novel transcription-based isothermal in situ amplification system as well as with reverse transcription-polymerase chain reaction. D[1A] receptor protein was distributed throughout theatrium and ventricular myocardium. Preimmune and preabsorption controls were negative. Electron microscopic immunocytochemistry using the protein A gold method demonstrated the D[1A] receptor along the cellular membranes of coronary smooth muscle cells and ventricular myocytes and in the myosin thick filaments and M-lines. D[1A] receptor mRNA was present in coronary vessels and myocardium in amplified but not in unamplified sections. Western blot analysis showed specific D[1A] bands in transfected LTK{-} cells and the atrium but not in nontransfected LTK{-} cells and the ventricle. The selective L1-like receptor agonist SKF38393 stimulated adenylyl cyclase in ventricular myocardial plasma membranes in a dose-related fashion, and the response was abolished by the selective D1-like receptor antagonist SCH23390. These results demonstrate that the D[1A] receptor gene and protein are expressed in normal rat heart. The physiological and pathophysiological roles and predominant cell signaling mechanism or mechanisms of this receptor remain to be determined

ГРНТИ	34.41.35

ВИНИТИ 341.41.35.21.09
Рубрики: СЕРДЦЕ
ДОФАМИНОВЫЕ РЕЦЕПТОРЫ
СУБТИП 1А
ЭКСПРЕССИЯ
КРЫСЫ

Доп.точки доступа:
Ozono, Ryoji; O'Connell, Damian P.; Vaughan, Carl; Botkin, Suzanne J.; Walk, Scott F.; Felder, Robin A.; Carey, Robert M.

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