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РЖ ВИНИТИ 34 (BI12) 95.09-04Б2.209

Kristufek, D.
Regulation of 'бета'-xylosidase formation by xylose in Trichoderma reesei [Text] / D. Kristufek, S. Zeilinger, C. P. Kubicek // Appl. Microbiol. and Biotechnol. - 1995. - Vol. 42, N 5. - P713-717 . - ISSN 0175-7598
Перевод заглавия: Регуляция образования 'бета'-ксилозидазы [культурой] Trichoderma reesei ксилозой
Аннотация: The soft-rot fungus Trichoderma reesei forms 'бета'-xylosidase (EC 3.2.1.37) activity during cultivation on xylan and xylose, but not on glucose. When mycelia precultivated on glycerol were washed and transferred to fresh medium without a carbon and nitrogen source, 'бета'-xylosidase formation was induced by xylan, xylobiose and xylose. A supply of 4 mM xylose and a pH of 2,5 provided optimal conditions for induction. 'бета'-Xylosidase accounted for the major portion of total extracellular protein under these conditions, and could be purified to physical homogeneity by a single anion exchange chromatography step. A recombinant strain of T. reesei that carries multiple copies of the homologous xylanase II-encoding gene has a six-fold increased xylanase activity, but forms comparable 'бета'-xylosidase activities. This shows that the rate of xylan hydrolysis has no effect on the induction of 'бета'-xylosidase. Methyl-'бета'-D-xyloside inhibited 'бета'-xylosidase competitively and was a weak 'бета'-xylosidase inducer. The induction by xylobiose and xylan was strongly enhanced by the simultaneous addition of methyl-'бета'-D-xyloside and xylan or xylobiose. The results suggest that a slow supply of xylose is a trigger for 'бета'-xylosidase induction. Австрия, Abteilung Biochem., Inst. Biochem. Technol. und Mikrobiol., TU Wien. Getreidemarkt 9/1725, A-1060 Vienna. Библ. 29

ГРНТИ	34.27.17

ВИНИТИ 341.27.17.09.15
Рубрики: КСИЛОЗИДАЗА 'БЕТА'
ОБРАЗОВАНИЕ
РЕГУЛЯЦИЯ КСИЛОЗОЙ
TRUCHODERMA REESEI

Доп.точки доступа:
Zeilinger, S.; Kubicek, C.P.

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