СибНСХБ

Вид поиска

Область поиска

Найдено в других БД

Формат представления найденных документов:
библиографическое описание	краткий	полный

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>S=ОБРАБОТКА ВИТРИФИКАЦИОННЫМ РАСТВОРОМ<.>)

Общее количество найденных документов : 2
Показаны документы с 1 по 2

РЖ ВИНИТИ 34 (BI49) 96.01-04М1.23

Fuku, Eiji.
In vitro viability and ultrastructural changes in bovine oocytes treated with a vitrification solution [Text] / Eiji Fuku, Jianmin Liu, Bruce R. Downey // Mol. Reprod. and Dev. - 1995. - Vol. 40, N 2. - P177-185 . - ISSN 1040-452X
Перевод заглавия: In vitro жизнеспособность и ультраструктурные изменения в ооцитах крупного рогатого скота, обработанных витрификационным раствором
Аннотация: Abattoir-derived oocytes were exposed to a concentrated cryoprotectant solution (DAP213:2 M DMSO, 1 M acetamide, 3 M propanediol, and 10% FCS in TCM199) for 1.5 or 5 min at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Their viability was assessed by in vitro fertilization (IVF) and culture (IVC) to blastocysts. To investigate the effect of DAP213 on the ultrastructure, GV and IVM oocytes were processed for transmission electron microscopy (TEM) before (control) or after exposure to the cryoprotectant. DAP213 induced profound ultrastructural modifications to the microvilli and mitochondria, resulted in large vesicle formation, and, most significantly, caused the premature release of the cortical granules (CG). In IVM oocytes exposed to the cryoprotectant for 5 min, exocytosis of CG into the perivitelline space was common and the IVF raet was reduced (P.05). After exposure for 5 min, GV oocytes displayed clusters of CG comparable to controls, but after IVM-IVF, polyspermy rate was increased (P.05). Furthermore, treated GV oocytes showed a reduced rate of cleavage and blastocyst formation and an increased percentage of oocytes exhibiting alterations in organelles, whereas the viability and ultrastructure of IVM oocytes treated for 1.5 min was not different from controls. These observations demonstrate that 1) cortical granule kinetics is one of the key elements controlling fertilizability of bovine oocytes treated with cryoprotectant, and 2) GV oocytes are more sensitive to the cryoprotectant than those that have already been matured in vitro. Канада, Dep. of Aminal Sci., Macdonald Campus, McGill Univ., Quebec

ГРНТИ	34.21.15

ВИНИТИ 341.21.15.09.15
Рубрики: ЯЙЦЕКЛЕТКИ
ООЦИТЫ
ОБРАБОТКА ВИТРИФИКАЦИОННЫМ РАСТВОРОМ
ЖИЗНЕСПОСОБНОСТЬ IN VITRO
КРС

Доп.точки доступа:
Liu, Jianmin; Downey, Bruce R.

РЖ ВИНИТИ 34 (BI05) 96.02-04И8.257

Fuku, Eiji.
In vitro viability and ultrastructural changes in bovine oocytes treated with a vitrification solution [Text] / Eiji Fuku, Jianmin Liu, Bruce R. Downey // Mol. Reprod. and Dev. - 1995. - Vol. 40, N 2. - P177-185 . - ISSN 1040-452X
Перевод заглавия: In vitro жизнеспособность и ультраструктурные изменения в ооцитах крупного рогатого скота, обработанных витрификационным раствором
Аннотация: Abattoir-derived oocytes were exposed to a concentrated cryoprotectant solution (DAP213:2 M DMSO, 1 M acetamide, 3 M propanediol, and 10% FCS in TCM199) for 1.5 or 5 min at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Their viability was assessed by in vitro fertilization (IVF) and culture (IVC) to blastocysts. To investigate the effect of DAP213 on the ultrastructure, GV and IVM oocytes were processed for transmission electron microscopy (TEM) before (control) or after exposure to the cryoprotectant. DAP213 induced profound ultrastructural modifications to the microvilli and mitochondria, resulted in large vesicle formation, and, most significantly, caused the premature release of the cortical granules (CG). In IVM oocytes exposed to the cryoprotectant for 5 min, exocytosis of CG into the perivitelline space was common and the IVF raet was reduced (P.05). After exposure for 5 min, GV oocytes displayed clusters of CG comparable to controls, but after IVM-IVF, polyspermy rate was increased (P.05). Furthermore, treated GV oocytes showed a reduced rate of cleavage and blastocyst formation and an increased percentage of oocytes exhibiting alterations in organelles, whereas the viability and ultrastructure of IVM oocytes treated for 1.5 min was not different from controls. These observations demonstrate that 1) cortical granule kinetics is one of the key elements controlling fertilizability of bovine oocytes treated with cryoprotectant, and 2) GV oocytes are more sensitive to the cryoprotectant than those that have already been matured in vitro. Канада, Dep. of Aminal Sci., Macdonald Campus, McGill Univ., Quebec

ГРНТИ	34.39.57

ВИНИТИ 341.39.57.87.13.02
Рубрики: ЯЙЦЕКЛЕТКИ
ООЦИТЫ
ОБРАБОТКА ВИТРИФИКАЦИОННЫМ РАСТВОРОМ
ЖИЗНЕСПОСОБНОСТЬ IN VITRO
КРС

Доп.точки доступа:
Liu, Jianmin; Downey, Bruce R.

"Электронные каталоги и базы данных библиотек СО РАН"

© Международная Ассоциация пользователей и разработчиков электронных библиотек и новых информационных технологий
(Ассоциация ЭБНИТ)

rtvarBDview(" результаты поиска","20190607010228","4682442");

Вид поиска