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РЖ ВИНИТИ 34 (BI39) 96.11-04К1.351

Harris, C. L.
Characterization of a glycosyl-phophatidylinositol anchor-deficient subline of Raji cells. An analysis of the functional importance of complement inhibitors on the Raji cell line [Text] / C. L. Harris, B. P. Morgan // Immunology. - 1995. - Vol. 86, N 2. - P311-318 . - ISSN 0019-2805
Перевод заглавия: Характеристика дефицитных по гликозил-фосфатидилинозитольному якорю сублинии клеток Raji. Анализ функционального значения ингибиторов комплемента на клеточной линии Raji
Аннотация: Analysis of complement inhibitory proteins present on the surface of Raji cells originally established from human Burkitt's lymphoma revealed two populations of cells. These populations differed in their expression of the glycosyl-phosphatidylinositol (GPI)-anchored inhibitors CD59 and decay-accelerating factor (DAF). Two stable clones were established by limiting dilution of the original cell culture. Raji + 3 expressed CD59 and DAF whereas Raji - 26 expressed neither inhibitor. Both clones expressed membrane cofactor protein (MCP). Analyses of other cell surface proteins (CD19, CD35, CD48 and CD58) revealed similar levels of expression of transmembrane proteins by both clones. However, CD48 was expressed only by Raji + 3. As CD48, DAF and CD59 are all GPI-anchored molecules it is likely that a defect in the GPI-anchoring mechanism is responsible for the generation of the second population of cells. The two clones demonstrated markedly different sensitivities to complement. When equally sentisized cells from both clones were treated with normal human serum (12*5%) for 1 hr at 37'ГРАДУС', the Raji + 3 clone was resistant to complementmediated lysis, whereas approximately 70% of the Raji - 26 cells were lysed. However, by using specific antibody to block the function of membrane-bound complement inhibitors, lysis of Raji + 3 was demonstrated. Whilst blocking of one inhibitor only on the cell had little effect on cell killing, blocking of two or more inhibitors significantly increased cell lysis. However, whilst a limited protective role was seen for MCP, CD59 and DAF appeared to be of far more importance for protection from complement-mediated lysis via the classical pathway. Великобритания, Dep. Med. Biochem., Univ. Wales Med. College, Cardiff. Библ. 31

ГРНТИ	34.43.21

ВИНИТИ 341.43.21.09
Рубрики: КОМПЛЕМЕНТ
ЛИТИЧЕСКОЕ ДЕЙСТВИЕ НА КЛЕТКИ
СВЯЗЬ С КЛЕТОЧНЫМИ ИНГИБИТОРАМИ
КУЛЬТИВИРУЕМЫЕ КЛЕТКИ RAJI

Доп.точки доступа:
Morgan, B.P.

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