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РЖ ВИНИТИ 34 (BI24) 96.06-04М3.21

Oztas, Baria.
Intracarotid hypothermic saline infusion: A new method for reversible blood-brain barrier disruption in anesthetized rats [Text] / Baria Oztas, Mutlu Kucuk // Neurosci. Lett. - 1995. - Vol. 190, N 3. - P203-206 . - ISSN 0304-3940
Перевод заглавия: Внутрикаротидная гипотермическая инфузия солевого раствора: новый метод обратимого раскрытия гемато-энцефалического барьера у наркотизированных крыс
Аннотация: An animal model for reversible blood-brain barrier disruption has been developed. Retrograde infusion of hypothermic saline solution (8'+-'1'ГРАДУС'C) into the left external carotid artery of normothermic, Wistar rats reversibly increases cerebrovascular permeability to Evans blue albumin in the left cerebral hemisphere. Isotonic saline solutions at 37'ГРАДУС'C for Group I and at 8'+-'1'ГРАДУС'C for Group II were infused for 30 s at a constant rate of 0.12 ml/s into the left external carotid artery. Evans blue, the barrier tracer, was administered intravenously either prior to or at intervals 5, 30, 180, 360 min after the hypothermic saline infusion under pentobarbital anesthesia. All animals receiving hypothermic saline perfusion had disturbed blood-brain barrier permeability. Based on visual inspection, disruption grade in the left hemispheres of 10 of 16 animals was 3+. Mean values for Evans blue dye were found to be 0.32'+-'0.08 mg% in left the hemisphere after normothermic saline infusion (Group I), and 2.9'+-'0.4 mg% in the same hemisphere after hypothermic saline infusion (Group II). The difference was found to be significant between Group I and Group II (P 0.001). The increase in cerebrovascular permeability was temporary, however, although Evans blue albumin extravasion remained sligtly elevated 3 h after infusion, it was normal 6 h after infusion

ГРНТИ	34.39.15

ВИНИТИ 341.39.15.17
Рубрики: ГЕМАТО-ЭНЦЕФАЛИЧЕСКИЙ БАРЬЕР
ПРОНИЦАЕМОСТЬ
ОБРАТИМЫЕ НАРУШЕНИЯ
МЕТОДЫ
ИНФУЗИЯ ГИПОТЕРМИЧЕСКОГО РАСТВОРА
КРЫСЫ

Доп.точки доступа:
Kucuk, Mutlu

РЖ ВИНИТИ 34 (BI25) 96.10-04М5.48

Oztas, Baria.
Intracarotid hypothermic saline infusion: A new method for reversible blood-brain barrier disruption in anesthetized rats [Text] / Baria Oztas, Mutlu Kucuk // Neurosci. Lett. - 1995. - Vol. 190, N 3. - P203-206 . - ISSN 0304-3940
Перевод заглавия: Внутрикаротидная гипотермическая инфузия солевого раствора: новый метод обратимого раскрытия гемато-энцефалического барьера у наркотизированных крыс
Аннотация: An animal model for reversible blood-brain barrier disruption has been developed. Retrograde infusion of hypothermic saline solution (8'+-'1'ГРАДУС'C) into the left external carotid artery of normothermic, Wistar rats reversibly increases cerebrovascular permeability to Evans blue albumin in the left cerebral hemisphere. Isotonic saline solutions at 37'ГРАДУС'C for Group I and at 8'+-'1'ГРАДУС'C for Group II were infused for 30 s at a constant rate of 0.12 ml/s into the left external carotid artery. Evans blue, the barrier tracer, was administered intravenously either prior to or at intervals 5, 30, 180, 360 min after the hypothermic saline infusion under pentobarbital anesthesia. All animals receiving hypothermic saline perfusion had disturbed blood-brain barrier permeability. Based on visual inspection, disruption grade in the left hemispheres of 10 of 16 animals was 3+. Mean values for Evans blue dye were found to be 0.32'+-'0.08 mg% in left the hemisphere after normothermic saline infusion (Group I), and 2.9'+-'0.4 mg% in the same hemisphere after hypothermic saline infusion (Group II). The difference was found to be significant between Group I and Group II (P 0.001). The increase in cerebrovascular permeability was temporary, however, although Evans blue albumin extravasion remained sligtly elevated 3 h after infusion, it was normal 6 h after infusion

ГРНТИ	34.39.05

ВИНИТИ 341.39.05
Рубрики: ГЕМАТО-ЭНЦЕФАЛИЧЕСКИЙ БАРЬЕР
ПРОНИЦАЕМОСТЬ
ОБРАТИМЫЕ НАРУШЕНИЯ
МЕТОДЫ
ИНФУЗИЯ ГИПОТЕРМИЧЕСКОГО РАСТВОРА
КРЫСЫ

Доп.точки доступа:
Kucuk, Mutlu

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