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РЖ ВИНИТИ 34 (BI49) 96.10-04Я6.233

Peral, Maria J.
Cytoslic pH regulation in chicken enterocytes: Na{+}-independent regulatory cell alkalinization [Text] / Maria J. Peral, M.Luisa Calonge, Anunciacion A. Ilundain // Biochim. et biophys. acta. Biomembranes. - 1995. - Vol. 1233, N 1. - P84-88 . - ISSN 0005-2736
Перевод заглавия: Регуляция цитозольного pH в энтероцитах курицы. Na{+}-независимая регуляция защелачивания клеток
Аннотация: The mechanisms involved in intracellular pH (pH[i]) recovery from an acid load have been investigated in enterocytes isolated from chicken. Following an intracellular acidification, by abrupt withdrawal of NH[4]Cl, pH[i] alkalinized in the nominally absence of Na{+} and bicarbonate. This Na{+}- and bicarbonate-independent (NBI) regulatory cell alkalinization became negligible when the pH[i] has reached a value of approx. 6.85. Addition of Na{+} induced a rapid pH[i] recovery to control values. Ronenone, DCCD, vanadate, NBD-Cl, SCH 28080 and EIPA inhibited the NBI cell alkalinization, whereas bafilomycin A[1], ouabain and H[2]-DIDS were without effect. Na{+}-dependent pH[i] recovery from an acid load was inhibited by EIPA and unaffected by SCH 28080 or DCCD. The rate of NBI cell alkalinization was a linear function of the electrochemical proton gradient. In high external K{+} buffer plus valinomycin the line goes through the origin. Gramicidin accelerated the rate of NBI cell alkalinization, whereas it was slightly reduced by low external potassium. The results demonstrate that in intestinal epithelial cells exist at least two mechanisms for proton secretion: a Na{+}-H{+} exchanger and a Na{+}- and bicarbonate-independent proton transport system. This latter mechanism appears to be a proton conductance pathway. Испания, Dep. Fisiol., Biol. Anim. Fac. Farm., Univ. Sevilla. Библ. 27

ГРНТИ	34.19.19

ВИНИТИ 341.19.19.19
Рубрики: НАТРИЙ
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ЗАЩЕЛАЧИВАНИЕ КЛЕТОК
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Доп.точки доступа:
Calonge, M.Luisa; Ilundain, Anunciacion A.

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