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РЖ ВИНИТИ 34 (BI39) 96.05-04К1.209

Quantitative assessment of the specifec CD4+ T lymphocyte proliferative response in bovine herpesvirus 1 immune cattle [Text] / M. Denis [et al.] // Vet. Immunol. and Immunopathol. - 1994. - Vol. 42, N 3-4. - P275-286 . - ISSN 0165-2427
Перевод заглавия: Количественная оценка пролиферативного ответа специфических CD4{+} T-лимфоцитов у иммунных коров проти вируса герпеса типа 1 быка
Аннотация: Were quantified the CD4{+} T cell proliferation specific for bovine herpesvirus 1 (BHV-1) in peripheral blood mononuclear cells from cattle. By using proliferative assays performed after negative selection were shown that, as expected, CD4{+} T cells were the limiting cell type for antigen-induced proliferation. Neither B, 'гамма''дельта' T nor CD8{+} cells seemed to be involved. The limiting dilution method was established to obtain quantitative estimations, namely frequencies of specific T cells. When limiting dilution cultures were supplemented with interleukin-2 (IL-2), an IL-2 induced unspecific cell proliferation masked the specific T cell proliferation. Natural killer cells were not the major cell type involved, but CD4{+} lymphocytes themselves seemed to respond to IL-2 irrespective of the presence of antigen. When cultures were performed without addition of IL-2, the frequency of BHV-1 specific proliferative T cells could be obtained by the difference between the frequency of proliferating cells calculated in the presence and absence of antigen. The method provides a sensitive and quantitative means to measure the T cell immune response to BHV-1 vaccine candidates. Бельгия, Fac. Veterinary, Univ. Liege. Библ. 15

ГРНТИ	34.43.29

ВИНИТИ 341.43.29.11
Рубрики: ЛИМФОЦИТЫ T CD4{+}
ПРОТИВОВИРУСНЫЕ
ПРОЛИФЕРАЦИЯ IN VITRO
ВИРУС ПРОСТОГО ГЕРПЕСА КОРОВ
КОРОВЫ

Доп.точки доступа:
Denis, M.; Kaashoek, M.J.; Oirschot, J.T.van; Pastoret, P.-P.; Thiry, E.

РЖ ВИНИТИ 34 (BI05) 96.06-04И8.129

Quantitative assessment of the specifec CD4+ T lymphocyte proliferative response in bovine herpesvirus 1 immune cattle [Text] / M. Denis [et al.] // Vet. Immunol. and Immunopathol. - 1994. - Vol. 42, N 3-4. - P275-286 . - ISSN 0165-2427
Перевод заглавия: Количественная оценка пролиферативного ответа специфических CD4{+} T-лимфоцитов у иммунных коров против вируса герпеса типа 1 быка
Аннотация: Were quantified the CD4{+} T cell proliferation specific for bovine herpesvirus 1 (BHV-1) in peripheral blood mononuclear cells from cattle. By using proliferative assays performed after negative selection were shown that, as expected, CD4{+} T cells were the limiting cell type for antigen-induced proliferation. Neither B, 'гамма''дельта' T nor CD8{+} cells seemed to be involved. The limiting dilution method was established to obtain quantitative estimations, namely frequencies of specific T cells. When limiting dilution cultures were supplemented with interleukin-2 (IL-2), an IL-2 induced unspecific cell proliferation masked the specific T cell proliferation. Natural killer cells were not the major cell type involved, but CD4{+} lymphocytes themselves seemed to respond to IL-2 irrespective of the presence of antigen. When cultures were performed without addition of IL-2, the frequency of BHV-1 specific proliferative T cells could be obtained by the difference between the frequency of proliferating cells calculated in the presence and absence of antigen. The method provides a sensitive and quantitative means to measure the T cell immune response to BHV-1 vaccine candidates. Бельгия, Fac. Veterinary, Univ. Liege. Библ. 15

ГРНТИ	34.39.57

ВИНИТИ 341.39.57.13.19
Рубрики: ЛИМФОЦИТЫ T CD4{+}
ПРОТИВОВИРУСНЫЕ
ПРОЛИФЕРАЦИЯ IN VITRO
ВИРУС ПРОСТОГО ГЕРПЕСА КОРОВ
КОРОВЫ

Доп.точки доступа:
Denis, M.; Kaashoek, M.J.; Oirschot, J.T.van; Pastoret, P.-P.; Thiry, E.

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