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РЖ ВИНИТИ 34 (BI49) 96.02-04Я6.29

Rols, Marie-Pierre.
Pulse-first heterofusion of cells by electric field pulses and associated loading of macromolecules into mammalian cells [Text] / Marie-Pierre Rols, Francoise Dahhou, Justin Teissie // BioTechniques. - 1994. - Vol. 17, N 4. - P762-764, 766-767 . - ISSN 0736-6205
Перевод заглавия: Гетерослияние клеток одиночным электрическим импульсом и введение макромолекул в клетки млекопитающих
Аннотация: Exposing cells to brief, high-intensity electrical field pulses can lead to the permeabilization of their plasma membranes. This electro-induced permeated state of the cell membrane is reversible and leads to cell fusion; i. e., the electropermeabilized state is fusogenic. The size of cells intended for fusing, however, limits the obtention of viable hybrids when there is a wide range of cell sizes. We report here that electrofusion of cells of different origins and sizes (hamster fibroblasts and red blood cell ghosts) can be easily obtained. Due to size differences, the optimum electric field conditions for their permeabilization and the maintenance of their integrity are observed to be different (10 pulses, 100 'мю's duration, 1.2 kV/cm for Chinese hamster ovary [CHO] cells and 10 pulses, 100 'мю's duration, 3.6 kV/cm for erythrocyte ghosts). Cells and ghosts are electropermeabilized separately and kept at low temperature. Their fusion is then induced by the creation of contact between them by gentle centrifugation. This process takes advantage of the longlived fusogenicity of the electropermeabilized cell membrane. An immediate consequence is the introduction of macromolecules into mammalian cells. This is obtained by fusing cells with pre-loaded erythrocyte ghosts. Under optimum conditions, penetration of large quantities of FITC-dextran (70 kDa) and 'бета'-galactosidase into 90%-95% of CHO cells while preserving their viability has been observed. Франция, CNRS, Toulouse. Библ. 26

ГРНТИ	34.19.05

ВИНИТИ 341.19.05.27
Рубрики: КЛЕТКИ
ГЕТЕРОСЛИЯНИЕ ОДИНОЧНЫМ ЭЛЕКТРИЧЕСКИМ ИМПУЛЬСОМ
МАКРОМОЛЕКУЛЫ
ВВЕДЕНИЕ В КЛЕТКИ МЛЕКОПИТАЮЩИХ

Доп.точки доступа:
Dahhou, Francoise; Teissie, Justin

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