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РЖ ВИНИТИ 34 (BI39) 96.04-04К1.171

Irie, Hanna Yoko.
CD8'бета' chain influences CD8'альфа' chain-associated Lck kinase activity [Text] / Hanna Yoko Irie, K. S. Ravichandran, Steven J. Burakoff // J. Exp. Med. - 1995. - Vol. 181, N 4. - P1267-1273 . - ISSN 0022-1007
Перевод заглавия: Цепь CD8'бета' обусловливает связанную с CD8'альфа' цепью активность Lck киназы
Аннотация: The CD8 molecule plays an important role in the differentiation of CD8{+} T cells in the thymus and in their normal function in the periphery. CD8 exists on the cell surface in two forms, the 'альфа''альфа' homodimer and the 'альфа''бета' heterodimer. Recent studies indicate an important role for the CD8'бета' chain in thymic development of CD8{+} T cells and suggest that signaling via CD8'альфа''бета' may be distinct from CD8'альфа''альфа'. To better understand these differences, we introduced the CD8'бета' gene into a T cell hybridoma which only expressed the CD8'альфа''альфа' homodimer. In the parent hybridoma, cross-linking of the CD8'альфа' chain led to minimal enhancement of CD8-associated Lck tyrosine kinase activity. In the CD8'бета'{+} transfectants, several observations suggested that CD8'бета' modifies CD8'альфа'-associated Lck tyrosine kinase activity: (a) in in vitro kinase assays, antibodymediated crosslinking of CD8 alone, or CD8 cross-linking with the TCR, resulted in 10-fold greater activation of Lck kinase activity, compared to cells expressing CD8'альфа''альфа' alone; (b) in vivo, markedly enhanced tyrosine phosphorylation of several intracellular proteins was observed upon CD8 cross-linking with the TCR in CD8'альфа''бета'-expressing cells, compared to cells expressing CD8'альфа''альфа' alone; and (c) Lck association with CD8'альфа' was stabilized by the coexpression of CD8'бета'. Thus, the differential Lck kinase activation and tyrosine phosphorylation seen with CD8'альфа''альфа' vs. CD8'альфа''бета' may reflect the unique signaling capabilities of the CD8'бета' molecule. These differences in signaling may, in part, account for the diminished ability to generate CD8 single positive thymocytes in mice bearing a homozygous disruption of the CD8'бета' gene. США, Dana-Farber Cancer Inst., Boston, MA02115. Библ. 29

ГРНТИ	34.43.29

ВИНИТИ 341.43.29.07
Рубрики: ЛИМФОЦИТЫ Т CD8{+}
ПОВЕРХНОСТНЫЕ МАРКЕРЫ
АКТИВНОСТЬ КИНАЗЫ LCK
ГИБРИДОМНЫЕ КЛОНЫ

Доп.точки доступа:
Ravichandran, K.S.; Burakoff, Steven J.

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