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РЖ ВИНИТИ 34 (BI14) 96.04-04Б4.232

Garduno, A. Rafael
Physical and functional S-layer reconstitution in Aeromonas salmonicida [Text] / A.Rafael Garduno, Barry M. Phipps, William W. Kay // J. Bacteriol. - 1995. - Vol. 177, N 10. - P2684-2694 . - ISSN 0021-9193
Перевод заглавия: Физическое и функциональное изменение S-слоя у Aeromonas salmonicida
Аннотация: The various function attributed to the S-layer of Aeromonas salmonicida have been previously identified by their conspicuous absencer in S-layer-defective mutants. As a different approach to establish the multifunctional nature of this S-layer, we established methods for reconstitution of the S-layer of A.salmonicida. Then we investigated the functional competence of the reconstituted S-layer. S-layers were reconstituted in different systems: on inert membranes or immobilized lipooplysaccharide (LPS) from purified S-layer protein (A-protein) or on viable cells from either A-protein or preassembled S-layer sheets. In the absence of divalent cations and LPS, purified A-protein in solution spontaneously assembled into tetrameric oligomers and, upon concentration by ultrafiltration, into macroscopic, semicrystalline sheets formed by oligomers loosely organized in a tetragonal arrangement. In the presence of Ca{2+}, purified A-protein assembled into normal tetragonal arrays of interlocked subunits. A-protein bound with high affinity (K[d], 1.55'ЭКВИВ'10{-7} M) and specificity to high-molecular-weight LPS from A.salmonicida but not to the LPSs of several other bacterial spesies. In vivo, A-protein could be reconstituted only on A.salmonicida cells which contained LPS, and Ca{2+} affecd both a regular tetragonal organization of the reattachem A-protein and an enhanced reattachment of the A-protein to the cell surface

ГРНТИ	34.27.29

ВИНИТИ 341.27.29.07.13.09
Рубрики: AEROMONAS SALMONICIDA (BACT.)
S-СЛОЙ
ТРАНСФОРМАЦИЯ
МУТАНТЫ
А-ПРОТЕИНЫ
ЛИПОПОЛИПРОТЕИНЫ

Доп.точки доступа:
Phipps, Barry M.; Kay, William W.

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