СибНСХБ

Вид поиска

Область поиска

Найдено в других БД

Формат представления найденных документов:
библиографическое описание	краткий	полный

Поисковый запрос: (<.>S=ЧУВСТВИТЕЛЬНОСТЬ KCA(2+)<.>)

Общее количество найденных документов : 1

РЖ ВИНИТИ 34 (BI48) 96.12-04Т2.33

MCI-154 increases Ca{2+} sensitivity of reconstituted thin filament. A study using a novel in vitro motility assay technique [Text] / Masataka Sata [et al.] // Circ. Res. - 1995. - Vol. 76, N 4. - P626-633 . - ISSN 0009-7330
Перевод заглавия: MCI-154 повышает чувствительность к Ca{2+} тонких мышечных волокон, воссозданных [из актина и тропонин-тропомиозинового комплекса]. Изучение с помощью нового метода определения подвижности
Аннотация: MCI-154 (6-[4-(4'-pyridylamino)phenyl]-4,5-dihydro-3(2H)pyridazinone hydrochloride trihydrate) is a potent novel cardiotonic agent whose positive inotropism is shown to be mainly based on an increase in Ca{2+} sensitivity of the contractile apparatus. To elucidate the exact mechanism through which this drug acts, we investigated the movement of the reconstituted thin filament on a myosin layer in vitro. Cardiac thin filaments were reconstituted from actin and tropomyosin-troponin complex purified from rat cardiac acetone powder separately. Double staining of the filament showed that tropomyosin-troponin complex was integrated along actin filament homogeneously. Thin filaments thus prepared were fluorescently labeled and made to slide on rat cardiac myosin fixed on a glass coverslip while varying the [Ca{2+}] of the medium (control, pH 7.2 at 25'ГРАДУС'C). When [Ca{2+}] was low, the filaments showed only brownian motion. However, above a certain level of [Ca{2+}] (the threshold [Ca{2+}]), the filaments started to slide, and the velocity increased, reaching the maximum velocity within a very narrow range of [Ca{2+}]. The regulation was completely abolished by using simple actin filaments without tropomyosin-troponin complex, demonstrating that the regulatory proteins are responsible for this Ca{2+} regulation of the movement of the reconstituted thin filament. Under the control condition, addition of MCI-154 shifted the threshold [Ca{2+}] to a lower level (sensitization) in a concentration-related manner. And 10{-4} mol/L of MCI-154 reversed the desensitization effect induced by either acidosis (pH 6.8), low temperature (15'ГРАДУС'C), or the addition of inorganic phosphate (10 mmol/L). However, the maximum sliding velocity was not affected by the drug under any condition. In conclusion, MCI-154 directly sensitized the contractile apparatus under not only physiological but also pathophysiological conditions. Библ. 47

ГРНТИ	34.45.21

ВИНИТИ 341.45.21.29.11.11.17
Рубрики: КАРДИОТОНИЧЕСКИЕ СРЕДСТВА
MCI-154
ТОНКИЕ МЫШЕЧНЫЕ ВОЛОКНА СЕРДЦА
ЧУВСТВИТЕЛЬНОСТЬ KCA(2+)
НОВЫЙ МЕТОД ОПРЕДЕЛЕНИЯ

Доп.точки доступа:
Sata, Masataka; Sugiura, Seiryo; Yamashita, Hiroshi; Fujita, Hideo; Momomura, Shin-ichi; Serizawa, Takashi

"Электронные каталоги и базы данных библиотек СО РАН"

© Международная Ассоциация пользователей и разработчиков электронных библиотек и новых информационных технологий
(Ассоциация ЭБНИТ)

rtvarBDview(" результаты поиска","20190607010228","4682442");

Вид поиска