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РЖ ВИНИТИ 34 (BI13) 95.09-04Б3.45

Doran, Joy B.
Saccharification and fermentation of sugar cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway [Text] / Joy B. Doran, H. C. Aldrich, L. O. Ingram // Biotechnol. and Bioeng. - 1994. - Vol. 44, N 2. - P240-247 . - ISSN 0006-3592
Перевод заглавия: Осахаривание и ферментация богассы сахарного тростника с помощью Klebsiella oxytoca P2, содержащего хромосомно встроенные гены, кодирующие матаболический путь этанола у Zymomonas mobilis
Аннотация: Pretreatment of sugar cane bagasse is essential for a simultaneous saccharification and fermentation (SSF) process wich uses recombinant Klebsiella oxytoca strain P2 and Genencor Spezyme CE, Strain P2 has been genetically engineered to express Zymomonas mobilis genes encoding the ethanol pathway and retains the native ability to transport and metabolize cellobiose (minimizing the need for extracellular cellobiase). In SSF studies with this organism, both the rate of ethanol production and ethanol yield were limited by saccharification at 10 and 20 filter paper units (FPU) g{-1} acid-treated bagasse. Dilute slurries of biomass were converted to ethanol more efficiently (over 72% of theoretical yield) in simple batch fermentations than slurries containing high solids, ableit with the production of lower levels of ethanol. With high solids (i.e., 160 g acid-treated bagasse L{-1}), a combination of 20 FPU cellulase g{-1} bagasse, preincubation under saccharification conditions, and additional grinding (to reduce particle size) were required to produce ca. 40g ethanol L{-1}. Alternatively, almost 40 g ethanol L{-1} was produced with 10 FPU cellulase g{-1} bagasse by incorporating a second saccharification step (no further enzyme addition) followed by a second inoculation and short fermentation. In this way, a theoretical ethanol yield of over 70% was achieved with the production of 20 g ethanol 800 FPU{-1} of commercial cellulase. США, Dept Microbiol. and Cell Sci., Univ. Florida, Gainesville, Florida, 32611. Библ. 34

ГРНТИ	34.27.39

ВИНИТИ 341.27.39.09.29
Рубрики: ЖТИНОЛ
ПОЛУЧЕНИЕ
ФЕРМЕНТАЦИЯ
БАГАССА САХАРНОГО ТРОСТНИКА
KLEBSIELLA OXYTOCA
РЕКОМБИНАНТНЫЙ ШТАММ P2

Доп.точки доступа:
Aldrich, H.C.; Ingram, L.O.

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