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РЖ ВИНИТИ 34 (BI39) 97.01-04К1.640

Selective growth of human mast cells induced by Steel factor, IL-6, and prostaglandin E[2] from cord blood mononuclear cells [Text] / Hirohisa Saito [et al.] // J. Immunol. - 1996. - Vol. 157, N 1. - P343-350 . - ISSN 0022-1767
Перевод заглавия: Избирательный рост тучных клеток человека, вызываемый фактором стали, интерлейкином (IL) 6 и простагландином (PG) E[2] из мононуклеарных клеток пупочной крови
Аннотация: To establish the method for generating a large number of mature human mast cells, were cultured cord blood mononuclear cells (CBMC) in several conditions in the presence of Steel factor (SF). Among several cytokines tested, IL-6 enhanced SF-dependent mast cell growth from purified CD34{+} cells for more than 8 wk in culture. When CBMC were cultured instead of CD34{+} cells, IL-6 enhanced the mast cell development in the presence but not in the absence of PGE[2]. PGE[2] enhanced the SF- and IL-6-dependent development of mast cells from CBMC probably by blocking granulocyte-macrophage CSF (GM-CSF) secretion from accessory cells, because PGE[2] or anti-GM-CSF enhanced the mast cell development induced by SF and IL-6 from CBMC, but not from CD34{+} cells; GM-CSF inhibited the enhancing effect of IL-6 on the mast cell development from CD34{+} cells; and PGE[2] inhibited GM-CSF secretion from CBMC. The mast cells cultured in the presence of SF, IL-6, and PGE[2] for10 wk were 99% pure, and seemed to be functionally mature, because they contained 5.62 'мю'g of histamine and 3.46 'мю'g of tryptase per 10{6} cells; and when sensitized with human IgE and then challenged with anti-human IgE, the cells released a variety of mediators such as histamine. Electron-microscopic analysis revealed that some of the cultured mast cells are morphologically mature. Япония, Nat. Children's Med. Res. Ctr., Tokyo. Библ. 43

ГРНТИ	34.43.51

ВИНИТИ 341.43.51.11.21
Рубрики: ТУЧНЫЕ КЛЕТКИ
МЕТОД ПОЛУЧЕНИЯ IN VITRO
КУЛЬТИВИРОВАНИЕ КЛЕТОК ПУПОЧНОЙ КРОВИ
ИНТЕРЛЕЙКИН 6
ПРОСТАГЛАНДИН E[2]
ФАКТОР СТАЛИ
ЧЕЛОВЕК

Доп.точки доступа:
Saito, Hirohisa; Ebisawa, Motohiro; Tachimoto, Hiroshi; Shichijo, Michitaka; Fukagawa, Kazumi; Matsumoto, Kenji; Likura, Yoji; Awaji, Takeo; Tsujimoto, Gozo; Yanagida, Makoto; Uzumaki, Hiroya; Takahashi, Gen; Tsuji, Koichiro; Nakahata, Tatsutoshi

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