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РЖ ВИНИТИ 34 (BI36) 95.11-04А4.336

On-line preparation of {1}{1}C-labelled acetate and palmilate by column extraction [Text] : abstr. 10th Int. Symp. Radiopharm. Chem., Kyoto, 1993 / R. Iwata [et al.] // J. Labell. Compound and Radiopharm. - 1994. - Vol. 35. - P134-135 . - ISSN 0362-4803
Перевод заглавия: Прямое получение меченных {1}{1}C ацетата и пальмитата с помощью колоночной экстракции
Аннотация: The on-line preparation of [{1}{1}C]acetate was carried out using the semi-automated system. [{1}{1}C]Carbon dioxide, concentrated in a cold trap cooled with liquid argon, was introduced with a He flow of 30 ml/min into a short reaction column packed with well dried Extrelut (Merck), loaded with 0.1-0.2 ml of commercially available 1М methylmagnesium bromide in THF. The Grignard reaction mixture was quenched by injecting 0.1 ml of 3N HCl into the reaction column and the [{1}{1}C]acetic acid formed was extracted with 5 ml of diethyl ether and transferred from the reaction column to a glass vessel. The ether was rinsed with He bubbling for 1 min to remove the unreacted [{1}{1}C]carbon dioxide and then passed through a subsequent Extrelut column absorbing 1 ml of a 7 w/v% sodium bicarbonate solution, where the [{1}{1}C]acetic acid was extracted back. It was finally eluted from the column with 10 ml of saline. For the preparation of [{1}{1}C]palmitate freshly prepared 1М n-pentadecylmagnesium bromide in THF and hexane were used instead of methylmagnesium bromide and diethyl ether. The [{1}{1}C]palmitic acid extracted in the same way was then adsorbed with a short silica gel column (Sep-Pak Silica, Waters) and eluted with 10 ml of ethanol. [{1}{1}C]acetate was obtained in overall radiochemical yields of over 80% by the present on-line preparation method within 10 min including the recovery and concentration steps of [{1}{1}C]carbon dioxide, while [{1}{1}C]palmitate was prepared in 30-40% radiochemical yields. HPLC analysis showed over 95% radiochemical purities for these [{1}{1}C]fatty acids. Япония, Cyclotron and Radioisotope Center and Inst. of Development, Aging and Cancer, Tohoku University, Aramaki, Aoba-ku, Sendai 980.

ГРНТИ	34.49.37

ВИНИТИ 341.49.37.13
Рубрики: РАДИОФАРМПРЕПАРАТЫ
УГЛЕРОД-11
АЦЕТАТ
ПАЛЬМИТАТ
КОЛОНОЧНАЯ ЭКСТРАКЦИЯ
ПОЛУАВТОМАТИЧЕСКАЯ СИСТЕМА

Доп.точки доступа:
Iwata, R.; Ido, T.; Takahashi, T.; Tada, M.

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