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РЖ ВИНИТИ 34 (BI39) 95.08-04К1.20

Starburst{TM} dendrimers: Enhanced performance and flexibility for immunoassays [Text] / Pratap Singh [et al.] // Clin. Chem. - 1994. - Vol. 40, N 9. - P1845-1849 . - ISSN 0009-9147
Перевод заглавия: Дендримеры Starburst {TM}. Увеличение возможностей и гибкости иммунологических проб
Аннотация: Starburst{TM} dendrimers are novel, water-soluble polymeris materials, with a well-defined composition and structure. In our application, we used dendrimers composed of poly(amidoamine) groups to which we coupled several specific antibodies, to investigate potential formats based on radial partition immunoassay. The coupled antibodies have retained their stability and immunological binding after coupling, both in solution and when immobilized onto a solid support. On the basis of our feasibility studies with model systems, we conclude that immunoassays can be developed with performance equivalent to or better than that in many established systems. By application of a mixture of the dendrimercoupled antibody and the analyte of interest to the solid phase, we have investigated the performance characteristics of solution-phase immunoassays. Our experiments demonstrate enhanced sensitivity for creatine kinase MB isoenzyme (CKMB), thyrotropin, and myoglobin assays and reduced instrumental analysis time for the CKMB assay

ГРНТИ	34.43.05

ВИНИТИ 341.43.05.07
Рубрики: ИММУНОЛОГИЧЕСКИЕ МЕТОДЫ
ОПРЕДЕЛЕНИЕ АНТИГЕНОВ
ДЕНДРИМЕР - НОВЫЙ СИНТЕТИЧЕСКИЙ МАТЕРИАЛ
СВОЙСТВА
ПРИМЕНЕНИЕ

Доп.точки доступа:
Singh, Pratap; Moll, Fred; Lin, Spencer H.; Ferzli, Charles; Yu, Kwok S.; Koski, R.Kent; Saul, Richard G.; Cronin, Peter

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