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РЖ ВИНИТИ 34 (BI49) 96.02-04Я6.132

Hajnoczky, Gjorgy.
Luminal communication between intracellular calcium stores modulated by GTP and the cytoskeleton [Text] / Gjorgy Hajnoczky, Chi Lin, Andrew P. Thomas // J. Biol. Chem. - 1994. - Vol. 269, N 14. - P10280-10287 . - ISSN 0021-9258
Перевод заглавия: ГТФ и цитоскелет модулируют сообщение между внутриклеточными кальциевыми депо
Аннотация: The activation properties of inositol 1,4,5-trisphosphate (InsP[3])-sensitive Ca{2+} stores in permeabilized and intact hepatocytes were investigated by monitoring Mn{2+} quench of fura-2 compartmentalized within these stores, as Mn{2+} passed through InsP[3]-activated channels in a retrograde manner. In cells permeabilized in suspension the InsP[3]-sensitive pool size was dependent on InsP[3] dose, and there was a large unresponsive compartment. By contrast, essentially all of the compartmentalized dye was accessible following activation of a small fraction of the InsP[3] receptors in carefully permeabilized attached cells. After loading the cytosol of intact hepatocytes with Mn{2+}, both submaximal and maximal vasopressin doses caused complete quench of the entire intracellular pool of compartmentalized fura-2. Vasopressin-induced Mn{2+} quench occurred in a stepwise manner at doses that gave cytosolic Ca{2+} oscillations, reflecting periodic opening of intracellular Ca{2+} channels. Pretreatment with thapsigargin to eliminate feedback effects of Ca{2+} fluxes converted the steps to a continuous quench. The data suggest that Ca{2+} stores in attached permeabilized and intact hepatocytes are luminally connected, making the entire store accessible to InsP[3]. In cells permeabilized in suspension, GTP increased InsP[3]-sensitive pool size, and this effect was inhibited by cytochalasin B. GTP did not change the initial rate of Mn{2+} quench but increased the proportion of slowly accessible stores in the InsP[3]-sensitive compartment, apparently by recruitment of InsP[3]-insensitive stores. Preincubation on ice or with cytoskeletal inhibitors dissociated slowly accessible compartments from the InsP[3]-sinsitive stores in both intact and subsequently permeabilized attached hepatocytes. Addition of GTP to permeabilized cells reversed this disruption of store continuity. It is suggested that GTP- and cytoskeleton-dependent luminal communication between Ca{2+} stores is an important determinant of function, which could modulate the availability of Ca{2+} for release. США, Dep. Pathol. Cell Biol., Thomas Jefferson Univ., Philadelphia, PA 19107. Библ. 50

ГРНТИ	34.19.17

ВИНИТИ 341.19.17.07.03.01
Рубрики: ГТФ
ЦИТОСКЕЛЕТ
МОДУЛЯЦИЯ
ВНУТРИКЛЕТОЧНЫЕ КАЛЬЦИЕВЫЕ ДЕПО

Доп.точки доступа:
Lin, Chi; Thomas, Andrew P.

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