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РЖ ВИНИТИ 34 (BI26) 96.02-04М4.368

H{+}-coupled 'альфа'-methylaminoisobutyric acid transport in human intestinal Caco-2 cells [Text] / David T. Thwaites [et al.] // Biochim. et biophys. acta. Biomembranes. - 1995. - Vol. 1234, N 1. - P111-118 . - ISSN 0005-2736
Перевод заглавия: Сопряженный транспорт H{+} и 'альфа'-метиламиноизомасляной кислоты в клетках кишечника человека Caco-2
Аннотация: Transepithelial apical-to-basal transport and cellular uptake of the non-metabolisable amino acid 'альфа'-methylaminoisobutyric acid (MeAIB) across confluent monolayers of the human intestinal epithelial cell line Caco-2 are enhanced by a transepithelial pH gradient (apical pH 6.0, basolateral pH 7.4). In Na{+}-free conditions (apical pH 7.4, basolateral pH74), net absorption (120 '+-' 58 pmol/cm{2} per h, n = 13) and uptake across the apical membrane (cell/medium ratio 0.56 '+-' 0.06, n = 13) are low. However, in Na{+}-free conditions with apical pH 6.0, net absorption (685 '+-' 95 pmol/cm{2} per h, n = 15) and intracellular accumulation (cell/medium ratio 3.63 '+-' 0.29, n = 14) were marked. Continuous monitoring of intracellular pH (pH[i]) in BCECF (2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein)-loaded Caco-2 cell monolayers indicated that apical addition of MeAIB (20 mM) was associated with H{+}-flow across the apical membrane in both Na{+} and Na{+}-free conditions. This transport process is rheogenic in Na{+}-free media, stimulating an inward short-circuit current in voltage-clamped Caco-2 cell monolayers. On the basis of competition for MeAIB accumulation and pH[i] experiments, L-proline, glycine, L-alanine and 'бета'-alanine are also substrates for H{+}-linked transport at the apical membrane of Caco-2 cells but L-valine, L-leucine and L-phenylalanine are not. These data are consistent with the expression, in the apical brush-border membrane of Caco-2 cells, of a H{+}-coupled, Na{+}-independent MeAIB carrier. Великобритания, Dep. Physiol. Sci., Univ. Newcastle upon Tyne, Med. Sch., Newcastle upon Tyne, NE2 4HH. Библ. 42

ГРНТИ	34.39.33

ВИНИТИ 341.39.33.27.02
Рубрики: ТРАНСПОРТ СОВМЕСТНЫЙ
ПРОТОНЫ
'АЛЬФА'-МЕТИЛАМИНОИЗОМАСЛЯНАЯ КИСЛОТА
КЛЕТКИ КИШЕЧНИКА CACO-2
ЧЕЛОВЕК

Доп.точки доступа:
Thwaites, David T.; McEwan, Gordon T.A.; Hirst, Barry H.; Simmuns, Nicholas L.

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