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РЖ ВИНИТИ 34 (BI39) 96.03-04К1.140

Автор(ы) : Garciandia, Ana, Lopez, Rafael, Tisire, Javier, Arrazola, Arantxa, Fortuno, Antonia, Bueno, Jose, diez, Javier
Заглавие : Enhanced Na{+}-H{+} exchanger activity and NHE-1 mRNA expression in lymphocytes from patients with essential hypertension
Источник статьи : Hypertension. - 1995. - Vol. 25, N 3. - С. 356-364
Аннотация: It has been demonstrated that the activity of the sodium-proton exchanger (NHE-1 isoform) is increased in lymphocytes and other blood cells from patients with essential hypertension. In the present study,we investigated whether an increased level of NHE-1-specific mRNA in lymphocytes from patients with essential hypertension would explain the enhanced transport activity. Twenty-two hypertensive patients and 21 normotensive subjects were studied. Basal cytosolic pH was measured by the pH-sensitive fluorescent probe 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Maximal sodiumproton exchange activity was determined by acidifying cell pH and measuring the initial rate of the net sodium-dependent proton efflux driven by an outward proton gradient. The transcript level of NHE-1 was measured by reverse transcription-polymerase chain reaction in comparison with a constitutively expressed reference gene ('бета'-actin). Intracellular pH was lower in hypertensive patients than normotensive subjects (7.34'+-'0.01 versus 7.39'+-'0.01, mean '+-'SEM, P .001). The maximal activity of the sodium-proton exchanger was higher in hypertensive patients than in normotensive subjects (1262'+-'100 versus 881'+-'56 mmol/L cells per hour, P.01). NHE-1 mRNA was increased in hypertensive patients compared with normotensive subjects (ratio of NHE-1 mRNA to 'бета'-actin mRNA, 0.16'+-'0.01 versus 0.12'+-'0.02, P.05). These data suggest that the increased sodium-proton exchange activity in essential hypertension may be related to the de novo synthesis of exchanger protein. Испания, Unidad Fisiopatol. Vascular, Dep. Med. Interna, Ctr. Invest. Biomed., Fac. Med., 31080 Pamplona. Библ. 51
ГРНТИ : 34.43.29
Предметные рубрики: ЛИМФОЦИТЫ
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