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РЖ ВИНИТИ 34 (BI39) 95.08-04К1.349

Автор(ы) : Fahnestock Margaret L., Dadgari Joseph M., McMillan, Minnie, Bjoekman Pamela J.
Заглавие : Phosphatidyl inositol-linked froms of a murine class I MHC molecule expressed on chinese hamster ovary cells retain peptide binding capability and alloreactivity
Источник статьи : Int. Immunol. - 1994. - Vol. 6, N 2. - С. 307-314
Аннотация: A gene encoding a phosphatidyl inositol-linked form of the murine class I MHC molecule H-2K{d} was constructed and the protein expressed in Chinese hamster ovary cells together with murine or human 'бета'[2]-microglobulin ('бета'[2]m). The resulting lipid-linked class I heterodimers can be efficiently converted into a soluble form by treatment of transfected cells with a phospholipase. Cells expressing K{d} heterodimers were characterized with respect to heavy chain levels at the cell surface, peptide binding, and recognition by K{d}-specific antibodies and alloreactive cytotoxic T cells. All transfectants bound a {3}H-labeled K{d}-restricted nonamer peptide, although more peptide bound to cells expressing the K{d}/human 'бета'[2]m combination, perhaps because of a greater number of empty molecules at the cell surface. A dissociation constant of 5*10{-8} M derived by Scatchard analysis is within the range expected for interactions of peptides with class I MHC molecules. Alloreactive cytotoxic T cells which recognize wild-type K{d} on murine cells lysed the hamster cells expressing lipid-linked K{d} without regard to the species of the 'бета'[2]m light chain. These results indicated that the engineered lipid-linked K{d} molecule is expressed at the cell surface, is recognized by antibodies and T cells, and functions to bind peptide. США, Howard Hughes Med. Inst., Pasadena, CA 91125. Библ. 55
ГРНТИ : 34.43.35
Предметные рубрики: АНТИГЕНЫ ГКГС
КЛАССА I
ЭКСПРЕССИЯ В КСЕНОГЕННЫХ КЛЕТКАХ
СОХРАНЕНИЕ СВОЙСТВ
МЫШИ
КИТАЙСКИЕ ХОМЯЧКИ
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