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Вид документа : Статья из журнала

РЖ ВИНИТИ 34 (BI49) 96.12-04М1.400

Автор(ы) : Ozono, Ryoji, O'Connell Damian P., Vaughan, Carl, Botkin Suzanne J., Walk Scott F., Felder Robin A., Carey Robert M.
Заглавие : Expression of the subtype 1A dopamine receptor in the rate heart : [Pap.] 49th Annu. Fall Conf. and Sci. Sess. Counc. High Blood Pressure Res., New Orleans, La, Sept. 16-22, 1995
Источник статьи : Hypertension. - 1996. - Vol. 27, N 3. - С. 693-703
Аннотация: In the present study using light microscopic immunohistochemistry, electron microscopic immunocytochemistry, and in situ amplification of mRNA, we demonstrate the D[1A] receptor in Sprague-Dawley and Wistar-Kyoto rat hearts. Selectivity was validated by recognition of the D[1A] receptor expressed in stably transfected LTK{-} cells. D[1A] receptor mRNA was detected with a novel transcription-based isothermal in situ amplification system as well as with reverse transcription-polymerase chain reaction. D[1A] receptor protein was distributed throughout theatrium and ventricular myocardium. Preimmune and preabsorption controls were negative. Electron microscopic immunocytochemistry using the protein A gold method demonstrated the D[1A] receptor along the cellular membranes of coronary smooth muscle cells and ventricular myocytes and in the myosin thick filaments and M-lines. D[1A] receptor mRNA was present in coronary vessels and myocardium in amplified but not in unamplified sections. Western blot analysis showed specific D[1A] bands in transfected LTK{-} cells and the atrium but not in nontransfected LTK{-} cells and the ventricle. The selective L1-like receptor agonist SKF38393 stimulated adenylyl cyclase in ventricular myocardial plasma membranes in a dose-related fashion, and the response was abolished by the selective D1-like receptor antagonist SCH23390. These results demonstrate that the D[1A] receptor gene and protein are expressed in normal rat heart. The physiological and pathophysiological roles and predominant cell signaling mechanism or mechanisms of this receptor remain to be determined
ГРНТИ : 34.41.35
Предметные рубрики: СЕРДЦЕ
ДОФАМИНОВЫЕ РЕЦЕПТОРЫ
СУБТИП 1А
ЭКСПРЕССИЯ
КРЫСЫ
Дата ввода:

Вид документа : Статья из журнала

РЖ ВИНИТИ 34 (BI23) 96.10-04М2.228

Автор(ы) : Ozono, Ryoji, O'Connell Damian P., Vaughan, Carl, Botkin Suzanne J., Walk Scott F., Felder Robin A., Carey Robert M.
Заглавие : Expression of the subtype 1A dopamine receptor in the rate heart : [Pap.] 49th Annu. Fall Conf. and Sci. Sess. Counc. High Blood Pressure Res., New Orleans, La, Sept. 16-22, 1995
Источник статьи : Hypertension. - 1996. - Vol. 27, N 3. - С. 693-703
Аннотация: In the present study using light microscopic immunohistochemistry, electron microscopic immunocytochemistry, and in situ amplification of mRNA, we demonstrate the D[1A] receptor in Sprague-Dawley and Wistar-Kyoto rat hearts. Selectivity was validated by recognition of the D[1A] receptor expressed in stably transfected LTK{-} cells. D[1A] receptor mRNA was detected with a novel transcription-based isothermal in situ amplification system as well as with reverse transcription-polymerase chain reaction. D[1A] receptor protein was distributed throughout theatrium and ventricular myocardium. Preimmune and preabsorption controls were negative. Electron microscopic immunocytochemistry using the protein A gold method demonstrated the D[1A] receptor along the cellular membranes of coronary smooth muscle cells and ventricular myocytes and in the myosin thick filaments and M-lines. D[1A] receptor mRNA was present in coronary vessels and myocardium in amplified but not in unamplified sections. Western blot analysis showed specific D[1A] bands in transfected LTK{-} cells and the atrium but not in nontransfected LTK{-} cells and the ventricle. The selective L1-like receptor agonist SKF38393 stimulated adenylyl cyclase in ventricular myocardial plasma membranes in a dose-related fashion, and the response was abolished by the selective D1-like receptor antagonist SCH23390. These results demonstrate that the D[1A] receptor gene and protein are expressed in normal rat heart. The physiological and pathophysiological roles and predominant cell signaling mechanism or mechanisms of this receptor remain to be determined
ГРНТИ : 34.39.29
Предметные рубрики: СЕРДЦЕ
ДОФАМИНОВЫЕ РЕЦЕПТОРЫ
СУБТИП 1А
ЭКСПРЕССИЯ
КРЫСЫ
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