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РЖ ВИНИТИ 34 (BI24) 96.04-04М3.7

Автор(ы) : Gao, Tian-Ming, Zou, Fei, Cheng, Pei-Xi
Заглавие : Свойства одиночных каналов NMDA-рецепторов нейронов мозговой коры
Источник статьи : Shengli xuebao. - 1995. - Vol. 47, N 2. - С. 133-141
Аннотация: The cell-attached and inside-out configurations of the patch-clamp techniques were used to investigate single channel properties of NMDA receptors in cultured intact neurons mechanically isolated from rat cereberal cortex. Recordings were made in Mg{2+}-free solutions. A channel, with a conductance of 35 pS was studied in detail with either NMDA or L-aspartate in the patch pipette. NMDA channels were permeableto Na{+} and K{+}, but not to Cl{-}, the mean open times and open probabilities of these channels were decreased with increasing hyperpolarization. Distributions fot the open times, closed times and burst durations required two-component fits. Channel openings were suppressed by APV. When Mg{2+} was included in the pipette, the mean open fimes were significantly diminished in a concentration- and voltage- dependent manner. Decreasing the bath temperature prolonged channel open times and decreased current amplitudes
ГРНТИ : 34.39.15
Предметные рубрики: НЕЙРОНЫ
КУЛЬТУРА
NMDA-РЕЦЕПТОРЫ
ОДИНОЧНЫЕ КАНАЛЫ
КЕРА БОЛЬШИХ ПОЛУШАРИЙ
КРЫСЫ
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